01441naa a2200301 a 450000100080000000500110000800800410001910000180006024501780007826000090025652005280026565000180079365000180081165000180082965000270084765000120087465000140088665000190090065000270091965000120094665000250095865300160098365300270099970000190102670000150104570000150106077300640107512786262024-05-21       1989    bl uuuu     u00u1 u    #d1 aPOLITO, V. S.  aOrigin of somatic embryos from repetitively embryogenic cultures of walnut (Juglans regia L.)bimplications for agrobacterium-mediated transformation.h[electronic resource]  c1989  aEarly stages of somatic embryo development from embryogenic cultures are described. Histological examination revealed that secondary somatic embryos arise from cotyledons and hypocotyls of primary embryos cultured in the dark. The embryos originate by transverse to oblique divisions of surface cells. Single-cell origin of the secondary embryos is thought to indicate the potential of the repetitive embryogenesis system for Agrobacterium-mediated transformation and regeneration of non-chimaeric transgenic walnut plants.  aAgrobacterium  abiotechnology  aembryogenesis  agenetic transformation  aJuglans  anut crops  atissue culture  avegetative propagation  awalnuts  aEngenharia Genética  aBroadleaves  aEmbriogenese somática1 aMcGRANAHAN, G.1 aPINNEY, K.1 aLESLIE, C.  tPlant Cell Reports, New Yorkgv. 8, n. 4, p. 219-221, 1989.