01658naa a2200217 a 450000100080000000500110000800800410001910000190006024500770007926000090015630000090016552009830017465000130115765300180117065300160118865300080120465300160121265300140122870000180124277301800126010267642010-03-12       1993    bl ---       0--  u    #d1 aCOLNAGO, L. A.  aStructure and dynamics of filamentous bacteriophages by solid state NMR.  c1993  ap.9.  aHigh resolution multinuclear and multidimensional solution NMR is an alternative to X-ray diffraction to solve the structure of proteins up to 30KDa. For large structures, solid state NMR has been used in several cases. The structural information obtained from solid state NMR spectroscopy is due to the angular dependence of the dipolar and quadrupolar interactions and chemical shift anisotropy. Solid state NMR was used to solve the strucuture and dynamics of filamentous bacteriophages class 1(M13, fd and f1) and class 2(pF1). The viruses are very usefull in molecular biology, as vectors. A new application of these bacteriophages resides in a fusion of peptides or proteins to theirs coat proteins, to create epitope libraries, vaccines, etc. We have been using N15, multidimensional solid state NMR to solve the structure of these fused peptides in the coat protein of gene VIII. Preliminary results show that only part of the fused peptides are structured in the virus.  adynamics  aBacteriophage  aFilamentous  aNMR  aSolid state  aStructure1 aOPELLA, S. J.  tIn: REUNIAO ANUAL DE FISICA DA MATERIA CONDENSADA, 22., maio 1993, Caxambu, MG. Programa e resumos... Sao Paulo: Sociedade Brasileira de Bioquimica e Biologia Molecular, 1993.