05516nam a2201201 a 450000100080000000500110000800800410001910000190006024500350007926000410011430000170015552027420017265000150291465000230292965000130295265000170296565000280298265000150301065000210302565000190304665000090306565000140307465000130308865000110310165000180311265000320313065000240316265000190318665000330320565000130323865000090325165000120326065000150327265000130328765000180330065000140331865000140333265000130334665000240335965000220338365000120340565000220341765000120343965000100345165000170346165000180347865000100349665000120350665000100351865000130352865000110354165000130355265000120356565000090357765000090358665000190359565000150361465300160362965300100364565300110365565300140366665300090368065300150368965300290370465300130373365300120374665300130375865300300377165300120380165300130381365300240382665300120385065300180386265300110388065300160389165300100390765300200391765300140393765300120395165300180396365300180398165300190399965300270401865300080404565300130405365300140406665300140408065300190409465300100411365300180412365300190414165300180416065300120417865300110419065300110420165300250421265300090423765300100424665300120425665300140426865300120428270000200429412489322004-09-24 1985 bl uuuu 00u1 u #d1 aDHINGRA, O. D. aBasic plant pathology methods. aBoca Raton, Florida: CRC Pressc1985 a355 p. cil. aSterlization of appartus and culture media; Heat sterlization; Gas sterilization; Filtration; Culture of pathogens; Factors affecting the cultivation and sporulation of pathogenic fungi; Some specialmethods of inducing sporulaion in fungi; Cultivation of pathogens for soil infestation; Culture media; Cultivation and sporulation; Genus and sporulation; Genus and species; Culture of bacteria; Long term storage of plantpathogens; lyophilization and vacuum drying; Detection and estimation of inoculum; Soilborne pathogens; Detection and estimation of onoculum in the air; Detction of Pathigens in seeds; Establishment of disease and testing for resistance; Factors influencing disease establishment;Root inoculation; Inoculation of stem; Inoculation of leaves; Inoculation of influrescence of cereal plants; Seed inoculation; Inoculation with bacteria; Inoculation of detached plant parts; Methodos of field inoculation; Some spcial hints; Isolation and enumeration of organisms in soil; Observation of soil microorganisms in Situ; Physiological Processes as an estimate of biological activity in soil; Spore germination in soil and soil fungistasis; Survival of plant pathogens in soil; Mycelial growth in soil; Collection of exudates from roots and Seeds; Artificial rhizosphere or Spermosphere; Chemical control; Spoore germination Test; Test based on growth on organisms in agar medium; Transfer bioassay tecniques; Laboratory Testing for soil fungicides; Greenhouse testing of Fungicides for control of soiborne pathogens; Seed treatment with chemicals; Collection and testing of soil samples for antogonists; Isolation of antibiotic producing organisms; Isolation and testing of lytic organisms; Isolation and Testing of mycoparasitic organisms; Spore perforating amoebae; Isolation of antagonists from leaf; Testing antogonists for biological control; Mounting and staining of fungi for microscopic examination; Marking of lysosmes and vacuoles in fungal spores; Mounting of spores for viewing from different positions; Immobilization of fungus spores in water mounts for microscopic; Staining of bacteria; Cements for Sealing mounts; Slide culture techniques and preparation of permanet mounts; Preparing agar cology mounts and dry reference cultures; Examination of host parasite relatonhip using light microscopy; Appendix a Culture media and their formulas; Appendix b plant nutrient solutions; Appendix c Solutions to maintain a constanthumidity in a closed atmosphere; Appendix d Dring agents; Appendix e pH indicator dyes; Appendix f Buffers; Appendix G Approximate figueres for concentrated acids and bases; Appendix H Glassware cleaning solutions; Appendix I Materia and techniques used for presevation of Wet specimens. aAlternaria abiological control aBotrytis acell culture aCeratocystis fagacearum aCercospora achemical control aColletotrichum acorn aCorticium aCoryneum acotton aculture media aCylindrocladium crotalariae ainoculation methods amicroorganisms aPlant diseases and disorders aresearch asoil astorage atechnology aAlgodão aArmazenamento aAscochyta aBactéria aBiologia aControle Biológico aControle Químico aDoença aDoença de Planta aFeijão aFungo aInoculação aMicrorganismo aMilho aMineral aÓleo aPesquisa aPlanta aQuímica aSemente aSoja aSolo aTecido Vegetal aTecnologia aAphanomices aBasic aBasico aBatatinha aBean aBiological aCephalosporium graminium aChemical aControl aControle aCultivo de microorganismo aDisease aDiseases aDiseases resistance aDoencas aFitopatologia aFungus aInoculation aMaize aMeio de cultivo aMethodods aMetodos aMicroorganims aMicroorganism aMicroorganismo aMicroorganismo do solo aOil aPathogen aPathology aPatologia aPhytopathology aPlant aPlant disease aPlant diseases aPlant sissues aPlantas aPlants aPotato aResistencia a doenca aSeed aSoils aSoybean aTechnique aTecnica1 aSINCLAIR, J. B.