01518naa a2200265 a 450000100080000000500110000800800410001910000240006024500860008426000090017052007450017965000160092465000180094065000140095865000230097265000170099565300180101265300320103065300250106270000190108770000190110670000170112570000170114277300930115912136092006-11-06       2005    bl uuuu     u00u1 u    #d1 aPICOLI, E. A. de T.  aIn vitro morphogenesis of Eucalyptus grandisbeffects of antibiotics on explants.  c2005  aThe effects of increasing cefotaxime, carbenicillin and timentin concentrations (0, 150, 300, 450 and 600 mg/l) on the in vitro morphogenesis of E. grandis explants were evaluated. Carbenicillin and timentin increased the frequency of explant regenerating calluses and decreased necrosis up to 600 mg l-1; cefotaxime behaved similarly up to 300 mg l-1. At this level there was an increasing frequency of explants and calluses showing necrosis. Hypocotyl and cotyledon explants presented similar morphogenetic results. The results suggest that carbenicillin or timentin should be used in Eucalyptus tissue culture protocols to control endophytic bacteria for genetic transformation protocols in view of their positive effect on callogenesis.  aantibiotics  amorphogenesis  aEucalipto  aEucalyptus Grandis  aMorfogênese  aAntibióticos  aCultura de tecidos vegetais  aPlant tissue culture1 aALFENAS, A. C.1 aDIAS, L. L. C.1 aNEVES, D. A.1 aOTONI, W. C.  tCrop Breeding and Applied Biotechnology, Viçosa, MGgv. 5, n. 2, p. 234-240, June 2005.