01668naa a2200241 a 450000100080000000500110000800800410001910000190006024500750007926000090015452009960016365000100115965000090116965000200117865000140119865300280121265300190124065300110125965300190127070000190128970000250130877300930133312136082006-11-06       2005    bl uuuu     u00u1 u    #d1 aFRANZON, R. C.  aIn vitro pollen germination of feijoa (Acca sellowiana (Berg) Burret).  c2005  aThe objective of this study was to establish a suitable culture medium and conditions to be used for in vitro germination tests of feijoa (A. sellowiana) pollen. It also aimed to test the possibility of storage of this pollen in a freezer (-18 degrees C). The pollen was collected from flowers immediately after flowering. The test media were the standard (10% sugar, plus 1% agar dissolved in distilled water), and the addition of two H3BO3 (0.65 mM and 1.3 mM) to this medium. Incubation temperatures of 25 and 30 degrees C were tested. A high average percentage of in vitro germination (79.7%) was obtained after three hours of incubation at 25 degrees C. The highest germination percentages (85.6, 52.3 and 9.3% after storage for zero, 90 and 150 days, respectively) were achieved with the addition of 1.3 mM of H3BO3 to the standard medium. Feijoa pollen lost viability after 90 and 150 days in freezer storage, reducing germination percentage from 79.7 to 45.9% and 6.1%, respectively.  aboron  aBoro  aMeio de Cultura  aMyrtaceae  aArmazenamento de pólen  aCulture medium  aFeijoa  aPollen storage1 aCORRÊA, E. R.1 aRASEIRA, M. do C. B.  tCrop Breeding and Applied Biotechnology, Viçosa, MGgv. 5, n. 2, p. 229-233, June 2005.