01672naa a2200289 a 450000100080000000500110000800800410001910000240006024501170008426000090020152007910021065000280100165000150102965000300104465000140107465300320108865300140112065300140113465300250114870000190117370000220119270000190121470000170123370000170125070000220126777300930128912136032006-11-06       2005    bl uuuu     u00u1 u    #d1 aPICOLI, E. A. de T.  aDetection and antibiotic treatment of Herbaspirillum huttiense isolated from in vitro explants of Eucalyptus sp.  c2005  aIn vitro assays involving explant cultures aiming to establish a genetic transformation protocol for the Eucalyptus grandis vs. E. smithii hybrid were hampered by the contamination of the explants. One yeast and one bacterium were isolated. The bacterium was identified as Herbaspirillum huttiense, with which further analyses were carried out and its response to different antibiotics were evaluated. Amongst 34 in vitro tested antibiotics, 12 inhibited the bacterial growth. Sulfadiazine, streptomycin, kanamycin and penicillin were selected for determination of the minimal inhibitory concentration. Sulfadiazine, streptomycin and kanamycin presented inhibition halos at 256 and 512 mg l-1, while penicillin did not display bacteriostatic effects in concentrations up to 1024 mg l-1.  abacterial contamination  aEucalyptus  aContaminação Bacteriana  aEucalipto  aCultura de tecidos vegetais  aEndófita  aEndophyte  aPlant tissue culture1 aALFENAS, A. C.1 aGONÇALVES, R. C.1 aDIAS, L. L. C.1 aNEVES, D. A.1 aOTONI, W. C.1 aROMEIRO, R. da S.  tCrop Breeding and Applied Biotechnology, Viçosa, MGgv. 5, n. 2, p. 191-198, June 2005.