02217naa a2200265 a 450000100080000000500110000800800410001902400510006010000230011124501490013426000090028352013800029265000150167265000230168765000270171065000140173765000230175165300160177465300190179065300280180965300080183770000170184570000210186277300680188312117002022-04-29       2002    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s00438-002-0665-62DOI1 aBRONDANI, R. P. V.  aTowards a genus-wide reference linkage map for Eucalyptus based exclusively on highly informative microsatellite markers.h[electronic resource]  c2002  aA novel set of 50 highly polymorphic microsatellite markers were developed and mapped on existing RAPD framework maps of Eucalyptus grandis and E. urophylla. Together with the twenty previously developed microsatellite markers, these were used to align the existing maps for the two most commercially important Eucalyptus species in the tropics. Sixty-three microsatellite markers were placed on the E. grandis map in 11 linkage groups, and 53 on the E. urophylla map distributed in 10 linkage groups. Approximately 66% of the microsatellite markers segregated in a fully informative fashion, allowing the establishment of colinear syntenic linkage groups between the two maps. The 50 new microsatellite markers were highly informative, with an average of 14 alleles per locus, and average expected heterozygosity between 0.82 and 0.87. Furthermore, within the subgenus Symphyomyrtus. to which the vast majority of commercially important Eucalyptus species belong, these markers display on average 90% transportability. This set of 70 mapped microsatellite markers represents a significant step toward the development of a genus-wide reference linkage map for Eucalyptus. These highly multiallelic and transportable markers constitute a powerful tool for QTL discovery and validation, and can be used in directed searches for QTL allele variation across Eucalyptus pedigrees.  aEucalyptus  aLinkage (genetics)  aMicrosatellite repeats  aEucalipto  aMarcador Molecular  aLinkage map  aMicrosatellite  aSimple sequence repeats  aSSR1 aBRONDANI, C.1 aGRATTAPAGLIA, D.  tMolecular Genetic Genomicsgv. 267, n. 3, p. 338-347, May 2002.