01513naa a2200205 a 450000100080000000500110000800800410001902400440006010000170010424501060012126000090022752008940023665000100113065000170114065000110115765300200116865300210118870000210120977300770123011993732022-09-15       1990    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/BF000334372DOI1 aREIFFERS, I.  aProduction of doubled haploid rice plants (Oryza sativa L.) by anther culture.h[electronic resource]  c1990  aThe results of anther culture of F 2 pollen issued from 23 single crosses are presented. A relation between the morphology of the panicle and the microspore stage was established. After cold-pretreatment (8 days at 4°C), the anthers were cultured on the callus-induction medium N6 supplemented with 1 mg 1-1 naphthaleneacetic acid. The calli were transferred to MS plant regeneration medium supplemented with 3mgl -~kinetin + 0.5 mg 1-' naphthaleneacetic acid. The induction frequency varied from 0.22% to 29% and the regeneration frequency from 0% to 144.4%, dependent upon the crosses used. On average, 27% of the plants obtained were albinos and 59% of the green plants underwent spontaneous chromosome doubling. Thirty- nine doubled haploid lines were evaluated and multiplied in the field. Lines with an excellent behaviour in upland culture conditions were selected from two crosses  aArroz  aOryza Sativa  aTecido  aDoubled Haploid  aField Evaluation1 aFREIRE, A. de B.  tPlant Cell, Tissue and Organ Culturegv. 21, n. 2, p. 165-170, May 1990.