02289naa a2200277 a 450000100080000000500110000800800410001902200140006010000220007424501970009626000090029352014720030265000100177465000160178465000080180065000120180865000120182065000200183265000230185265000110187565300160188670000170190270000170191970000190193677300560195511976332024-08-10       1993    bl uuuu     u00u1 u    #d  a0031-949X1 aGILBERTSON, R. L.  aGenetic diversity in geminiviruses causing bean golden mosaic diseasebthe nucleotide sequence of the infectious cloned DNA components of a brazilian isolate of bean golden mosaic geminivirus.  c1993  aThe complete nucleotide (nt) sequence of a Brazilian isolate of bean golden mosaic geminivirus (BGMV-BZ) was determined from the infectious cloned DNA components. The genome of BGMV-BZ was divided between two DNA components, DNA-A (2,617 nt) and DNA-B (2,580 nt), which had little sequence identity except for a 181-nt common region present in both molecules. The genomic organization of BGMV-BZ was similar to that of other bipartite whitefly-transmitted geminiviruses, with six open reading frames (ORFs) encoding putative proteins &gt10 kDa, four on DNA-A (one in the viral [+] and three in the viral complementary sense [–]) and two on DNA-B (one in the viral and one in the viral complementary sense). All six BGMV-BZ ORFs were analogous to corresponding ORFs in other whitefly-transmitted geminiviruses. The homology between BGMV-BZ sequences and those of a BGMV isolate from Puerto Rico (PR) were relatively low. BGMV-BZ sequences were no more closely related to BGMV-PR than to other distinct geminiviruses from South America, such as tomato golden mosaic geminivirus. Pseudo-recombinants made by mixing the infectious cloned DNAs of BGMV-BZ and a BGMV isolate from Guatemala (GA), which was very similar to BGMV-PR, were not infectious. These results support previous observations of differences in sap-transmissibility and bean genotype reactions between BGMV isolates and indicate that at least two distinct types of BGMV exist, designated type I and II.  aBeans  aNucleotides  aDNA  aDoença  aFeijão  aMosaico Dourado  aPhaseolus Vulgaris  aVírus  aGeminivirus1 aFARIA, J. C.1 aAHLQUIST, P.1 aMAXWELL, D. P.  tPhytopathologygv. 83, n. 7, p. 709-715, July 1993.