01896nam a2200241 a 450000100080000000500110000800800410001910000180006024500980007826001630017650000380033952011100037765000090148765000110149665300170150765300130152465300180153770000210155570000180157670000220159470000200161670000180163611893312025-07-08       2007    bl uuuu     u00u1 u    #d1 aMATSUMOTO, K.  aAgrobacterium-mediated transient gus gene expression in banana fruits.h[electronic resource]  aIn: ENCUENTRO LATINO AMERICANO Y DEL CARIBE DE BIOTECNOLOGIA AGROPECUARIA: REDBIO 2007, 6., 2007, Viña del Mar, Chile. Abstracts and posters? Roma: FAOc2007  aDisponível em formato de poster.  aTransient expression system of a reporter gene in banana fruits was developed for gene function as well as promoter studies. Mature and immature fruits of a triploid banana cvs. Nanicão and 'Nanica (Musa sp., AAA group, Cavendish subgroup) and a diploid hybrid (AA group, Embrapa/CNPMF 2803-01) were exposed to Agrobacterium tumefaciens strain LBA4404. In mature fruits, the bacteria, whose plasmid contains the gus-intron gene under the control of the CaMV 35S promoter, were suspended in a liquid medium with acetosyringone and infiltrated into the fruits by using a syringe. In immature fruits, they were vacuum-infiltrated into the sliced fruits and co-cultivated in liquid or semi-solid MS medium. GUS histochemical assay was realized three days after the syringe-infiltration or cocultivation. In comparison with the sliced fruits, calli from the same banana varieties were also tested. Results showed the immature fruits were good material for transient assays. The transient expression system developed in this study may be useful for routine transient assays to validate in-fruit function genes.  aMusa  aBanana  aCultivação  aPromoter  aReporter gene1 aCOELHO, M. C. F.1 aCABRAL, G. B.1 aARAGÃO, F. J. L.1 aTEIXEIRA, J. B.1 aCASTRO, D. de