01250naa a2200193 a 450000100080000000500110000800800410001910000200006024501470008026000090022752006330023665000130086965000130088265000120089565000230090765000260093070000220095677300780097811859942005-08-24       1997    bl uuuu     u00u1 u    #d1 aSA, M. F. G. de  aMolecular cloning of bruchid (Zabrotes subfasciatus) alpha-amylase cDNA and interactions of the expressed enzyme with bean amylase inhibitors.  c1997  aIntroduction; Materials and methods: Insects, RNA extraction, Northern blot analysis, Larval extraction, Reverse transcript-polymerase chain reaction and cDNA cloning, Cells plasmid, recombinant virus and insect cell transfection, Analysis of expressed Zs alfa-amylase and amylase activity detection, Protein assay, Binding ability of the inhibitor (alfaAI-2) to expressed Zs alfa-amylase; Results: Cloning of the ZsAmy cDNA, Analysis of the transcript, ZsAmy encodes and active amylase, Inhibition of Zs-amylase by bean alfa-amylase inhibitiors, at 30°C, ZsAmy forms a complex with alfaAI-2, but not with alfaAI-1. Discussion.  aCaruncho  aClonagem  aFeijão  aPhaseolus Vulgaris  aZabrotes Subfasciatus1 aCHRISPEELS, M. J.  tInsect Biochemistry and Molecular Biologygv. 27, n. 4, p. 271-281, 1997.