01781naa a2200181 a 450000100080000000500110000800800410001910000140006024501600007426000090023452011730024365000200141665300100143665300210144665300300146770000180149777300840151511852982022-05-10       2005    bl uuuu     u00u1 u    #d1 aMEHTA, A.  aIdentification of differentially expressed genes of Xanthomonas axonopodis pv. citri by representational difference analysis of cDNAh[electronic resource]  c2005  aXanthomonas axonopodis pv. citri is a phytopathogenic bacterium responsible for citrus canker, a serious disease which causes severe losses in citriculture around the world. In this study we report the differential expression of X. axonopodis pv. citri in response to specific treatments by using Representational Difference Analysis of cDNA (cDNA RDA). cDNAs from X. axonopodis pv. citri cultured in the presence of leaf extract of the host plant (Citrus sinensis), in vivo, as well as in the complex medium were hybridized against cDNA of the bacterium grown in the minimal medium. Sequencing of the difference products obtained after the second and third hybridizations revealed a total of 37 distinct genes identified by homology searches in the genome of X. axonopodis pv. citri. These genes were distributed in different functional categories, including genes that encode hypothetical proteins, genes involved in metabolism, cellular processes and pathogenicity, and mobile genetic elements. Most of these genes are likely related to growth and/or acquisition of nutrients in specific treatments whereas others might be important for the bacterium pathogenicity.  aCancro Cítrico  acitri  aExtrato de folha  aXanthomonas axonopodis pv1 aROSATO, Y. B.  tGenetics and Molecular Biology, Ribeirão Pretogv. 28, n. 1, p. 140-149, 2005.