01692naa a2200325 a 450000100080000000500110000800800410001910000220006024501170008226000090019930000150020852007860022365000150100965000120102465000230103665300090105965300150106865300150108365300250109865300130112365300200113670000210115670000250117770000190120270000170122170000190123870000170125770000160127477300760129011710962007-10-19       1996    bl uuuu     u00u1 u    #d1 aARAGÃO, F. J. L.  aInheritance of foreign genes in transgenic bean (Phaseolus vulgaris L.) co-transformed via particle bombardment.  c1996  ap. 142-150  aExploiting the biolistic process we have generated stable transgenic bean (Phaseolus vulgaris L.) plants with unlinked and linked foreign genes. Co-transformation was conducted using plasmid constructions containing a fusion of the gus and neo genes, which were co-introduced with the methionine-rich 2S albumin gene isolated from the Brazil nut and the antisense sequence of AC1, AC2, AC3 and BC1 genes from the bean golden mosaic geminivirus. The results revealed a co-transformation frequency ranging from 40% to 50% when using unlinked genes and 100% for linked genes. The introduced foreign genes were inherited in a Mendelian fashion in most of the transgenic bean lines. PCR and Southern blot hybridization confirmed the integration of the foreign genes in the plant genome.  abiolistics  aFeijão  aPhaseolus Vulgaris  aBean  aBiolistica  aElectrical  aFeijão transgênico  aParticle  aTransgenic bean1 aBARROS, L. M. G.1 aBRASILEIRO, A. C. M.1 aRIBEIRO, S. G.1 aSMITH, F. D.1 aSANFORD, J. C.1 aFARIA, J. C.1 aRECH, E. L.  tTheoretical and Applied Genetics, Berlingv.93, n.l/2, p.142-150, l996.