02762naa a2200265 a 450000100080000000500110000800800410001910000160006024501670007626000090024330000110025252018180026365000280208165000120210965000100212165000110213165300100214270000160215270000160216870000170218470000260220170000260222770000180225377302250227111614982017-03-28       2007    bl ---       0--  u    #d1 aAREVALO, J.  aIsolation, identification and characterization of egg-parasitizing fungi from guava plants infested with Meloigogyne mayaguensis in Brazil.h[electronic resource]  c2007  ap. 40.  aRecently, Meloidogyne mayaguensis was detected causing serious economic damage in a commercial plantation of guava in Brazil. Considering the possibility to use biological control agents in combination with another biological tactic to keep the population of this pest below the economic level, infested root samples were taken in a commercial guava plantation in Northeast Brazil with the objective to isolate egg parasitic fungi. Pure monospore cultures of different isolates were obtained and identified by cultural andorphological characteristics. Mycelia growth and sporulation in artificial media were evaluated at four different temperatures (20, 24, 28, 32°C), and mass production of chlamydospores/conidia  was determined through a solid state fermentation in cereal grain bags. The fungi Pochonia chlamydosporia var. chlamydosporia, P. chlamydosporia var. catenulata, Paecilomyces lilacinus and Lecanicillium psalliotae were identified and deposited in the Entomopathogenic Fungi Culture Collection in Genetic Resources and Biotechnology, Embrapa. The P. lilacinus isolate was not considered in the characterization studies. The optimal growth and chlamydospore production for Pochonia chlamydosporia isolates were in the range of 24 and 28°C, whereas L. psalliotae grew better at extreme temperatures than P. chlamydosporia isolates, but its conidia production was significatively affected when temperature increased. At 19 days of solid state fermentation, the P. chlamydosporia isolates produced between 3,5 and 5,2 x 10  chlamydospores per gram colonized substrate, and L. psalliotae produced 8,65 x 10  conidia per gram colonized substrate. The potential of these isolates as biological control agents of M. mayaguensis are discussed. This project has been funded by CNPq-Brazil (480001/2005-07).  aMeloidogyne mayaguensis  aDoença  aFungo  aGoiaba  aGuava1 aHIDALGO, L.1 aMARTINS, I.1 aSOUZA, J. F.1 aCASTRO, J. M. da C. e1 aCARNEIRO, R. M. D. G.1 aTIGANO, M. S.  tIn: REUNIÓN ANUAL DE LA ORGANIZACIÓN DE NEMATÓLOGOS DE LOS TRÓPICOS AMERICANOS: ONTA, 39., 2007, Córdoba, Argentina. Programa y resúmenes...Córdoba: Organización de Nematólogos de los Trópicos Americanos, 2007.