01647naa a2200205 a 450000100080000000500110000800800410001910000190006024501220007926000090020152010480021065000160125865000090127465000220128365000170130565000120132265300360133470000150137077300560138510159312022-04-21 2007 bl uuuu u00u1 u #d1 aJONSSON, C. M. aIn vitro effect of agriculture pollutants and their joint action on Pseudokirchneriella subcapitata acid phosphatase. c2007 aAcid phosphatase plays important roles in algae metabolism such as availability and recycling of inorganic phosphate, autophagic digestive processes and fertilization. Chemicals released into the environment from agriculture activities may impair algae phosphatase activity. The aim of this work was to evaluate the in vitro effect of twenty-four organic compounds and six metals used as pesticides, or present as contaminants in sewage sludge, on the acid phosphatase activity extracted from Pseudokirchneriella subcapitata. Results demonstrated that only the linear surfactant alkyl benzenesulphonate (LAS) and the heavy metals Hg2+, Al3+ and Cu2+ markedly altered (P50%) the enzyme activity. Join action inhibition studies indicated that Hg2+ was more potent inhibitor than Al3+ or LAS, and that the Hg2++Al3+ and Hg2+ + LAS mixtures have, respectively, additive and slight antagonism effects. Copper, which demonstrated an activator effect when preincubated with the enzyme, behaved as a slight antagonist for the inhibitor effect of Hg2+. aAgrotóxico aAlga aImpacto Ambiental aMetal Pesado aToxidez aPseudokirchneriella subcapitata1 aAOYAMA, H. tChemosphere, Oxfordgv. 69, n. 6, p. 849-855, 2007.