01410naa a2200181 a 450000100080000000500110000800800410001910000180006024500870007826000090016552009520017465000110112665000120113765000080114965300150115765300120117277300440118411489692025-10-21       1969    bl uuuu     u00u1 u    #d1 aHAWKER, J. S.  aInsoluble invertase from grapesban artifact of extraction.h[electronic resource]  c1969  aBoth soluble invertase (supematant from 10,000 g centrifugation) and insoluble invertase (precipitated with cell-wall fraction) were obtained from sultana grape berries homogenized under a variety of conditions. Treatment of the insoluble fraction with borate buffer, pH 8,5, polyethylene glycol (Carbowax4000) or non-ionic detergents solubilized most of the invertase. Homogenates of grapeberries prepared in the presence of Carbowax 4000, non-ionic detergents or protein (bovine serum albumin) contained up to 100 per cent of the total invertase in the soluble formo It is suggested that insoluble invertase from grapes is an artifact of extraction caused by the formation of a tannin-protein complex and/or a protein-tannin-cellwall complex. Invertase in grapes is probably located in the cytoplasm or vacuoles of cells. In contrast to grapes, insoluble invertase from aged aseptic carrot disks or from com coleoptiles could not be solubilized.  aGrapes  aExtrato  aUva  aArtificial  aVideira  tPhytochemistrygv. 8, p. 337-344, 1969.