01723naa a2200277 a 450000100080000000500110000800800410001910000190006024500920007926000090017150001390018052008150031965000130113465000210114765000280116865000190119665000220121565000350123765000220127265000290129465000220132365000090134570000200135470000130137477300580138711325342018-10-16       1997    bl uuuu     u00u1 u    #d1 aMELO, N. F. de  aIn vitro cultivation on modal segments of the umbu tree [Spondias tuberosa (Arr.) Cam].  c1997  aEdição do Proceedings of the 3 International Symposium on in Vitro Culture and Horticultural Breeding, Jerusalem, Israel, oct. 1996.  aA study dealing with in vitro cultivation of the umbu tree [Spondias tuberosa (Arr.) Cam], a Brazilian native xilophyta fruit tree was carried out to develop an appropriate micropropagation method. Nodal segments from one-year old seedlings were collected, disinfected superficially and culture on MS (1962) basal medium supplemented with a regime of BAP (6-benzylamino-purine) and IBA (indole-butyric-acid). After one-month cultivation period, the in vitro response was evaluated. the best combination produced 2.2 axillary shoots/nodal segment on semi-solid cujlture medium contnaining 0.1 mg/liter BAP. It seemed thar IBA and high concentration of BAP inhibited the vegetative growth. Vitrification, callusing and polyphenol accumulation were also detected in certain concentration of the growth regulators.  abreeding  amicropropagation  aplant growth substances  atissue culture  aCultura de Tecido  aMelhoramento Genético Vegetal  aMicropropagação  aRegulador de Crescimento  aSpondias Tuberosa  aUmbu1 aTEIXEIRA, J. B.1 aFARI, M.  tActa Horticulturae, Leuvengn. 447, p. 535-537, 1997.