01133naa a2200229 a 450000100080000000500110000800800410001910000250006024501250008526000090021030000130021952004900023265000120072265000230073465000100075765000120076765000200077965300190079965300150081870000190083377300510085210131522019-12-12       1999    bl uuuu     u00u1 u    #d1 aCASSIOLATO, A. M. R.  aFiltration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum Rifai.h[electronic resource]  c1999  ap. 43-46  aThe isolation of genetic markers, like drug resistance and auxotrophy, is a laborious but important step in genetic research. The isolation of auxotrophic mutants of Trichoderma harzianum using the filtration enrichment technique was more effective than using the total isolation technique. Most of 12 auxotrophic mutants exhibited similar growth rate and higher sporulation when compared with the wild type, but only two mutants (TWS-410 and TW5-523) could grow in 500ug/L of benomyl.  abenomyl  abiological control  afungi  amutants  aplant pathogens  aCulture medium  aTechniques1 aMELO, I. S. de  tRevista de Microbiologiagv.30, p.43-46, 1999.