01884naa a2200193 a 450000100080000000500110000800800410001910000140006024501330007426000090020752012740021665000210149065000210151165000140153265000220154665300220156870000150159077300850160511311402019-06-17       1986    bl uuuu     u00u1 u    #d1 aLLOYD, G.  aCommercially feasible micropropagation of mountain laurel, kalmia latifolia, by use of shoot-tip culture.h[electronic resource]  c1986  aThe multiplication at rates feasible for commercial production of mountain laurel. Kalmia latifalia. by micropropagation using shoot-tip cultures has been demonstrated. Shoot-tip explants placed initially in liquid woody plant medium (WPM) supplemented with 4-16 !iM N6_(~2-isopentenyl)-adenine (2iP) produced axillary shoots by 1 to 2 months. These new shoots were excised from the original explant and placed on the same WPM solidified with agar. The resultant shoot mass was subcultured monthly. Actively multiplying shoot-tip cultures were produced within 6 months. A comparison of 7 concentrations of 2iP, varying from Oto 64 !iMo showed that a concentration of 8 !iM 2iP produced the greatest number of utilizatble shoots after 8 weeks in culture. Stock cultures were maintained or increased monthly by removing and subculturing shoots elongating from the basal mass. Thirty to forty utilizable shoots were harvested from each culture 6 to 8 weeks after the initial subculture. Multiplication rates of 8 to 10 times were readily achieved. Harvested shoots rooted with 73% success in 4 to 6 weeks when placed in a 100% peat medium in a high humidity chamber. After a period of acclimation. these plants can be treated like young seedlings in commercial production.  aKalmia latifolia  amicropropagation  aMeristema  aMicropropagação  aShoot tip culture1 aMcCOWN, B.  tProceedings of International Plant Propagation Societygv. 30, p. 421-427, 1986.