01866naa a2200241 a 450000100080000000500110000800800410001910000260006024501370008626000090022352011380023265000180137065000200138865000150140865000120142365300140143565300220144965300260147165300250149770000190152270000260154177300570156710127852017-04-18       1997    bl uuuu     u00u1 u    #d1 aSILVEIRA, F. Q. de P.  aCarbohydrate-hydrolysing enzyme activity production by solid-state cultures of Trichoderma harzianum strains.h[electronic resource]  c1997  aScreening of nine Trichoderma harzianum strains grown by solid-state fermentation on media containing wheat bran as the carbon source demonstrated that different carbohydrase activities were produced by most of the strains investigated. B-Glucosidase, B-xylosidase, pectinase and a-amylase activities were produced by all strains. a-Amylase, B-xylanase and B-glucosidase activities were the best yields obtained. B-Mannosidase, a-glucuronidase, carboxymethyl-cellulase and FPase activities were poorly produced. The best balance of enzyme activities was obtained from cultures of T.harzianum strains 4 and 8. The procedure for enzyme extraction (25 mM sodium acetate buffer, pH 5.0) of T.harzianum strain C was compared with a second method using the above buffer sol ution containing 0.1% tween 80 plus blending in a homogenizer. The results indicated that the best yield of specific activity was obtained from the former extraction procedure. Furthermore, the protein content obtained by using this extraction procedure was very low. Carboxymethyl-cellulase activity was only detected when the second extraction procedure was used.  aculture media  aelectrophoresis  aextraction  astrains  aCell wall  aEnzyme production  aProtein determination  aSolid-state cultures1 aMELO, I. S. de1 aFERREIRA FILHO, E. X.  tRevista de Microbiologiagv.28, n.3 p.152-156, 1997.