02513naa a2200313 a 450000100080000000500110000800800410001910000190006024501170007926000090019650001520020552014670035765000200182465000160184465000120186065000220187265000260189465000190192065000170193965000110195665000140196765300260198165300270200770000170203470000250205170000150207670000190209177300890211011258672018-06-07       2009    bl uuuu     u00u1 u    #d1 aBONA, C. M. de  aCitrus asymmetric somatic hybrids produced via fusion of gamma-irradiated and iodoacetamide-treated protoplasts.  c2009  aTítulo em português: Híbridos somáticos assimétricos de citros produzidos pela fusão de protoplastos irradiados e tratados com iodoacetamida.  aThe objective of this study was to produce citrus somatic asymmetric hybrids by fusing gamma-irradiated protoplasts with iodoacetamide-treated protoplasts. Protoplasts were isolated from embryogenic suspension cells of grapefruit (Citrus paradisi Macfad.) cultivars Ruby Red and Flame, sweet oranges (C. sinensis Osbeck) 'Itaboraí', 'Natal', Valencia', and 'Succari', from 'Satsuma' (C. unshiu Marcow.) and 'Changsha' mandarin (C. reticulata Blanco) and 'Murcott' tangor (C. reticulata x C. sinensis). Donor protoplasts were exposed to gamma rays and receptor protoplasts were treated with 3 mmol L-1 iodoacetamide (IOA), and then they were fused for asymmetric hybridization. Asymmetric embryos were germinated, and the resulting shoots were either grafted onto sour orange, rough lemon or 'Swingle' (C. paradisi x Poncirus trifoliata) x 'Sunki' mandarin rootstock seedlings, or rooted after dipping their bases in indol-butyric acid (IBA) solution. The products were later acclimatized to greenhouse conditions. Ploidy was analyzed by flow cytometry, and hybridity was confirmed by amplified fragment length polymorphism (AFLP) analysis of plantlet DNA samples. The best treatment was the donor-recipient fusion combination of 80 Gy-irradiated 'Ruby Red' protoplasts with 20 min IOA-treated 'Succari' protoplasts. Tetraploid and aneuploid plants were produced. Rooting recalcitrance was solved by dipping shoots' stems in 3,000 mg L-1 IBA solution for 10 min.  aGamma radiation  aGrapefruits  aOranges  aProtoplast fusion  aSomatic hybridization  aFruta Cítrica  aLaranja Doce  aPomelo  aRaio Gama  afusão de protoplasto  ahibridação somática1 aGOULD, J. H.1 aMILLER JUNIOR, J. C.1 aSTELLY, D.1 aLOUZADA, E. S.  tPesquisa Agropecuária Brasileira, Brasília, DFgv. 44, n. 5, p. 454-462, maio 2009