01945naa a2200325 a 450000100080000000500110000800800410001910000210006024500970008126000090017850001260018752009340031365000220124765000190126965000230128865000110131165000280132265000100135065000110136065000110137165300250138265300170140765300170142465300100144165300130145170000230146470000220148770000190150977300910152811228142018-07-18       2007    bl uuuu     u00u1 u    #d1 aCALEGARIO, R. F.  aCharacterization of Tomato yellow spot virus, a novel tomato-infecting begomovirus in Brazil  c2007  aTítulo em português: Caracterização do Tomato yellow spot virus, um novo begomovírus isolado de tomateiro no Brasil.  aThe objective of this work was the biological and molecular characterization of a begomovirus detected in São Joaquim de Bicas, Minas Gerais, Brazil, named TGV-[Bi2], by determining its host range, complete nucleotide sequence and phylogenetic relationships with other begomoviruses. Biological characterization consisted of a host range study using either sap inoculation or particle bombardment as inoculation methods. The yellow spot virus can infect plants in Solanaceae and Amaranthaceae, including economically importat crops as sweet pepper, and weeds as Datura stramonium and Nicotiana silvestris. For the molecular characterization, the full-length genome (DNA-A and DNA-B) was amplified, cloned and completely sequenced. Sequence comparisons and phylogenetic analyses indicated that TGV-[Bi2] constitutes a novel begomovirus species named Tomato yellow spot virus (ToYSV), closely related to Sida mottle virus (SiMoV).  amolecular biology  aBemisia Tabaci  aBiologia Molecular  aInseto  aLycopersicon Esculentum  aPraga  aTomate  aVírus  aAnálise biológicva  aGeminivírus  aMosca-branca  aToYSV  awhitefly1 aFERREIRA, S. de S.1 aANDRADE, E. C. de1 aZERBINI, F. M.  tPesquisa Agropecuária Brasileira, Brasília, DFgv. 42, n. 9, p. 1335-1343, set. 2007