01226nam a2200229 a 450000100080000000500110000800800410001910000230006024501460008326001740022930000190040350000400042252003850046265000180084765000120086565000230087770000200090070000220092070000180094270000160096070000200097610001192022-05-17       2006    bl uuuu     u01u1 u    #d1 aSILVA, M. C. M. da  aIn vitro molecular evolution of bean alpha-amylase inhibitors to investigate specificity of binding to alpha-amylases.h[electronic resource]  aIn: ANNUAL INTERNATIONAL CONFERENCE ON INTELLIGENT SYSTEMS FOR MOLECULAR BIOLOGY, 14.; ANNUAL AB3C CONFERENCE, 2., 2006, Fortaleza. [Proceedings...]. Detroit: ISCBc2006  aNão paginado.  aISMB, X-MEETING 2006. Poster LB-28.  aShort Abstract: Combined DNA shuffling and Phage?display are important techniques to generate large number of alpha-amylase inhibitor mutants in vitro in order to identify new molecules with improved activities towards specific insects. We present new mutants and discuss possible repercussion of mutations on protein stability and function. We also introduced the STING parameter  aAlpha-amylase  aFeijão  aPhaseolus vulgaris1 aTEIXEIRA, F. R.1 aCRUZ, C. C. M. da1 aTOGAWA, R. C.1 aNESHICH, G.1 aSA, M. F. G. de