02301naa a2200313 a 450000100080000000500110000800800410001910000240006024500660008426000090015050001200015952013780027965000100165765000120166765000170167965000110169665000200170765300180172765300190174565300220176465300070178665300160179365300180180965300170182770000180184470000180186270000180188077300890189811189922018-07-30       2006    bl uuuu     u00u1 u    #d1 aDANTAS, A. C. de M.  aEmbryo rescue from interspecific crosses in apple rootstocks.  c2006  aTítulo em português: Resgate de embriões a partir de cruzamentos interespecíficos em porta-enxerto de macieira.  aThe objetive of this work was to rescue immature embryos of apple rootstocks Malus prunifolia (Marubakaido) and Malus pumila (M9) after 40-60 days of pollination and to put them into MS culture media supplemented with agar (6 g L-1) and casein hydrolysate (500 mg L-1). Embryos originated from interspecific crosses and open pollination showed differences in the in vitro responses, depending on the female parent, the developmental stage of the embryo, and the culture medium composition. Embryos of the M. pumila rootstock, rescued within 40 days after pollination and put in culture medium supplemented with indolacetic acid (IAA), gibberellic acid (GA3), kinetin and maltose, resulted in a normal development of plantlets. However, embryos originating from hand-pollination, cultivated in medium supplemented with 14 µM IAA, 5 µM kinetin and 1.5 µM Ga3 (MS1), mainly those of M. prunifolia x M. pumila, showed a high percentage of rusted embryos (96.2%). Embryos from open pollination of M. prunifolia and M. pumila formed calluses. It was possible to identify the influence of the female parent by the enhanced development of M. pumila shoots derived from open or hand-pollination. The crossing of responsive species and the use of the technique of embryo culture provided a rapid and uniform germination and, consequently, the development of fully normal seedlings  aMalus  aCarbono  aFruticultura  aMaçã  aMeio de Cultura  acarbon source  aculture medium  afontes de carbono  aM9  aMarubakaido  aPorta-enxerto  aPropagação1 aBONETI, J. I.1 aNODARI, R. O.1 aGUERRA, M. P.  tPesquisa Agropecuária Brasileira, Brasília, DFgv. 41, n. 6, p. 969-973, jun. 2006