02211naa a2200289 a 450000100080000000500110000800800410001902200150006002400540007510000140012924501490014326000090029252012860030165000210158765000110160865000150161965000360163465000190167065000180168970000220170770000190172970000310174870000310177970000200181070000220183077300690185221852732026-03-10 2025 bl uuuu u00u1 u #d a0329-8922.7 ahttps://doi.org/10.36995/j.recyt.2025.43.0062DOI1 aPERON, T. aChemical stability, viability, and in vitro gastrointestinal resistance of the probiotic bacillus in organic mango juice.h[electronic resource] c2025 aAbstract Probiotic juices must ensure their survival after processing and in the gastrointestinal tract to achieve their benefits. This study was divided into two stages. Initially, the objective was to evaluate the pH and viability of Bacillus clausii and Bacillus coagulans BC30 in organic mango juices subjected to heat treatment and, subsequently, to simulated accelerated shelf-life conditions. In the second stage, the pH, acidity, total soluble solids, the viability of B. clausii, as well as its resistance to the in vitro gastrointestinal tract (GIT) digestion were evaluated. In the first stage, at all analyzed times, the juice with B. clausii presented a lower pH than the juice with B. coagulans. The B. coagulans count decreased during the accelerated shelf-life test, whereas the B. clausii count remained stable. In the second stage, the lowest count of B. clausii was observed in the juice subjected to simulated industrial heat treatment (96 °C/35 s). However, as regards the other treatments (non-heat-treated and 80°C/10 min), the probiotic count was > 106 CFU/mL. An excellent performance of B. clausii was found in the juice that was not heat-treated and stored at different temperatures (5°C and 25°C) after the GIT test, with counts of > 107 CFU/mL. aBacillus clausii aJuices aShelf life aTropical and subtropical fruits aFruta Tropical aSuco de Fruta1 aSANTOS, R. A. dos1 aMARTINS, M. L.1 aLEITE JÚNIOR, B. R. de C.1 aBENEVENUTO, W. C. A. do N.1 aVIANA, E. de S.1 aMARTINS, E. M. F. tRevista De Ciencia Y Tecnologíagv. 27, n. 43, p 72–80, 2025.