01731naa a2200289 a 450000100080000000500110000800800410001910000160006024501380007626000090021450001510022352007260037465000120110065000210111265000130113365000220114665300270116865300140119565300260120970000210123570000210125670000200127770000200129770000200131770000150133777300890135211183842023-03-30 2006 bl uuuu u00u1 u #d1 aPACHECO, G. aInfluence of substrates and in vitro preconditioning treatments on ex vitro acclimatization of Arachis retusa.h[electronic resource] c2006 aNotas Científicas. Título em português: Influência de substratos e de pré-tratamentos in vitro na aclimatização ex vitro de Arachis retusa. aThe objective of this work was to evaluate the influence of substrate and preconditioning treatments on the acclimatization of in vitro plants of Arachis retusa. Plants were transferred to Plantmax or sand, and fertilized with Hoagland's nutrient solution. Plants maintained in sand, with or without fertilizer, showed the highest survival rates. In order to evaluate the influence of in vitro preconditioning treatments, stem segments were cultured on MS medium supplemented with different sucrose concentrations. The highest survival and developmental rates were observed in plants from two accessions cultured on MS supplemented with 1.5% and 3% sucrose. Flowering and fruit production were observed after five months. aArachis amicropropagation aAmendoim aMicropropagação aconservação in vitro agroundnut ain vitro conservation1 aGAGLIARDI, R. F.1 aCOGLIATTI, M. B.1 aMANHÃES, H. B.1 aCARNEIRO, L. A.1 aVALLS, J. F. M.1 aMANSUR, E. tPesquisa Agropecuária Brasileira, Brasília, DFgv. 41, n. 1, p. 165-169, jan. 2006