02939naa a2200217 a 450000100080000000500110000800800410001910000160006024501140007626000090019052022950019965000240249465000220251865000220254065000190256270000190258170000220260070000230262270000170264577300590266221821162026-04-06       2026    bl uuuu     u00u1 u    #d1 aBATISTA, I.  aCrop sequence effects on the genesis of soil aggregates in no-tillage systemsba focus on biogenic structures  c2026  aCrop diversification promotes soil fauna activity and aggregation, but the effect of crop type and sequencing on plant-soil organism interactions and their role in aggregate formation remains unclear. This study tested the hypothesis that crop rotation diversification under no-tillage is the primary factor supporting the quality and quantity of the available plant resources to soil macrofauna, thereby increasing the organism’s activity having as a consequence higher presence of biogenic aggregates compared to systems without rotation. Additionally, aim to identify which factors: crop sequence, seasonality (a rainy summer and a dry winter season) or aggregate type (physicogenic, intermediate or biogenic) are most directly associated with improvements of soil aggregates chemical and physical attributes. This study was conducted in a long-term (20 year) no-tillage corn-soybean crop rotation experiment at Jaboticabal, SP, Brazil (21◦14′ S, 48◦17′ W). The crop sequences included: soybean/corn rotation in the summer followed by corn in the winter; soybean/corn rotation in the summer followed by sunn hemp in the winter; soybean monoculture in the summer followed by sunn hemp in the winter; and corn monoculture in both the summer and winter growing seasons; additionally, two control areas were evaluated (Native Vegetation and Conventional Tillage). In each area at 0.0–0.1 m soil depth, soil aggregate types were identified as physicogenic, biogenic or intermediate. In each aggregate type mean diameter (WMD), geometric mean diameter (GMD), pH and Al, H++Al, Ca, Mg, P, and K content were determined. Also, total organic carbon (TOC), total nitrogen (N), particulate organic carbon (POC) and mineral-associated organic carbon (MAOC) were determined in each soil aggregate type. Biogenic aggregates showed superior structural stability, particularly during the dry season in treatments with corn and exhibited high organic carbon content across all fractions (TOC, POC, and MAOC). Grass/legume rotation did not positively influence aggregation; the presence of corn was a determining factor, likely due to its root architecture, which promotes the activity of soil “ecosystem engineers”. Overall, plants influence the processes that regulate aggregate formatio  aAggregate stability  aCropping sequence  aExposure pathways  aSoil structure1 aMACHADO, D. L.1 aCORREIA, M. E. F.1 aSPINELLI, M. H. M.1 aCORÁ, J. E.  tSoil & Tillage Researchgv. 257, , 106981, March 2026.