02281naa a2200325 a 450000100080000000500110000800800410001902200140006002400640007410000200013824501720015826000090033052012030033965000300154265000270157265000280159965000190162765000270164665000240167365000350169765000200173265000150175265300180176765300260178565300220181170000200183370000200185370000170187377300650189021816282025-11-18       2025    bl uuuu     u00u1 u    #d  a1472-765X7 ahttps://doi.org/https://doi.org/10.1093/lambio/ovaf1092DOI1 aZACARONI, A. B.  aNematicidal activity of Pochonia chlamydosporia blastospores and attempts to improve dehydration tolerance and storage stability with additives.h[electronic resource]  c2025  aAbstract: For the large-scale use of Pochonia chlamydosporia as a bionematicide, cost-effective production coupled with robust stabilization processes is essential. Submerged liquid fermentation yields large amounts of infective blastospores in a short period. This study aimed to evaluate the nematicidal activity of P. chlamydosporia blastospores from two fungal strains against the eggs of two plant-pathogenic nematodes, as well as to assess the use of various additives to enhance their dehydration tolerance and storage stability. For the more promising strain, submerged culture yielded 1 × 109 blastospores mL-1 after 96 h. Juvenile hatch inhibition was more pronounced with blastospores (64.8%–86.7%) than conidia (48.2%–65.3%) on Meloidogyne javanica and M. enterolobii eggs, respectively. Skim milk added to the liquid medium prior to inoculation or 72 h after fermentation protected fungal cells from dehydration. Following dehydration, storage stability after 10 days was significantly greater at 4°C compared to 25°C. Stabilization processes to prevent cell mortality during dehydration are crucial for the development of blastospores as active ingredients in bionematicides.  aBiological control agents  aNematicidal properties  aPochonia chlamydosporia  aSoil nematodes  aSubmerged fermentation  aControle Biológico  aFungo Para Controle Biológico  aMeloidogyne spp  aNematóide  aBionematicide  aDehydration tolerance  aStorage stability1 aMASCARIN, G. M.1 aFARIA, M. R. de1 aLOPES, R. B.  tLetters in Applied Microbiologygv. 78, n. 8, ovaf109, 2025.