02610naa a2200337 a 450000100080000000500110000800800410001902400380006010000200009824501490011826000090026752016110027665000220188765000220190965000200193165000240195165300160197565300170199165300140200865300170202270000190203970000210205870000200207970000170209970000230211670000180213970000200215770000220217770000200219977300530221921806222026-01-15       2026    bl uuuu     u00u1 u    #d7 a10.1016/j.cropro.2025.1074322DOI1 aCOSTA, I. B. da  aNeopestalotiopsis formicidarum causing leaf spots on Myciaria dubia, Eugenia stipitata and Psidium acutangulum in Brazil.h[electronic resource]  c2026  aCamu-camu (Myrciaria dubia), araçá-boi (Eugenia stipitata), and araçá-pera (Psidium acutangulum) are Myrtaceae species native to the Amazon region. Their fruits have multiple industrial applications, particularly in the food, cosmetic, and pharmaceutical sectors. Since 2020, leaf spot symptoms have been frequently observed on these fruit trees in both native and cultivated areas in northern Brazil. This study aimed to identify the etiological agent associated with leaf spots on camu-camu, araçá-boi and araçá-pera in the state of Roraima through morphological characterization and multilocus phylogenetic analysis. Twelve isolates of the genus Neopestalotiopsis were obtained from symptomatic leaf tissues. Based on morphological characters and phylogenetic analyses of the combined internal transcribed spacer (ITS) region, the transcription elongation factor 1-α (tef1) and partial sequences of the β-tubulin gene (tub2), the isolates were identified as Neopestalotiopsis formicidarum (syn N. formicarum). Three isolates were used in pathogenicity assay and inoculated into seedlings of M. dubia, E. stipitata, and P. acutangulum, causing small brown to black leaf spots that expanded and coalesced into larger necrotic lesions. This is the first report of N. formicidarum causing leaf spot on camu-camu, araçá-boi and araçá-pera in Brazil. Our study should be helpful for better understanding of the species N. formicidarum that represents a potential threat to the cultivation of these fruit trees, and will be useful for effective strategies for prevention and control this disease.  aNeopestalotiopsis  aEugenia Stipitata  aMyrciaria Dubia  aPsidium Acutangulum  aAraçá-boi  aAraçá-pera  aCamu-camu  aLeaf disease1 aMACHADO, J. F.1 aSACHET, M. T. G.1 aSILVA, G. F. da1 aSOUSA, T. F.1 aSILVA, I. J. S. da1 aCHAGAS, E. A.1 aCOSTA, T. E. da1 aHOLANDA, I. S. A.1 aMATOS, K. da S.  tCrop Protectiongv. 199, art. 107432, Jan. 2026.