02384naa a2200361 a 450000100080000000500110000800800410001902400570006010000200011724501090013726000090024652013340025565000150158965300220160465300280162665300260165465300250168065300230170565300150172865300170174370000200176070000200178070000170180070000180181770000210183570000210185670000150187770000240189270000280191670000180194470000210196277300390198321773762025-07-17       2025    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.phytochem.2025.1145492DOI1 aROCHA, K. A. D.  aBioactive withanolides from the leaves of Athenaea velutina (Sendtn.) D ’ Arcy.h[electronic resource]  c2025  aWithanolides are a substantial bio-diverse group of naturally occurring steroidal lactones. Herein, ten still undescribed withanolides (1 – 10), along with the knowns withaferin A (11), 2,3-dihidrowithaferin A (12), and two other non-withanolides, vomifoliol (13) and the N-trans-p-coumaroyltyramine (14), were isolated from the hexane/EtOAc 1:1 leaf extract of Athenaea velutina (Sendtn.) D ’ Arcy (Solanaceae). The structures of the unde- scribed withanolides were elucidated by an extensive analysis of their spectroscopic data: 1D and 2D NMR, HRESIMS, single-crystal X-ray diffraction, and ECD calculations. The antiproliferative properties of the with- anolides were evaluated against the human cancer cell lines: central nervous system (SNB-19), prostate (PC-3), colon (HCT-116), and leukemia (HL-60), and a murine fibroblast-like cell (L-929). Withanolides 15 – 17, isolated from the same plant in a previous work, were included for the pharmacological tests. Compounds 15 and 16 exhibited cytotoxic activity for all cancer cells, while 2 was selectively more cytotoxic to HL-60 cells. In addition, the withanolides were evaluated in guinea pig cardiac tissues. Compounds 15 and 16 showed cardiotonic ac- tivity, devoid of a positive chronotropic effect which is a good pharmacological profile for an inotrope agent.  aSolanaceae  aAthenaea velutina  aAtividade cardiotônica  aAtividade citotóxica  aCardiotonic activity  aCytotoxic activity  aWithaferin  aWithanolides1 aSILVEIRA, E. R.1 aPAULO, T. DE F.1 aAYALA, A. P.1 aSOARES, B. M.1 aMARANHÃO, S. S.1 aPESSOA, C. DO Ó1 aPAZ, I. A.1 aRODRIGUES, M. L. B.1 aNASCIMENTO, N. R. F. DO1 aCANUTO, K. M.1 aPESSOA, O. D. L.  tPhytochemistry, 238, 114549, 2025.