01950naa a2200205 a 450000100080000000500110000800800410001902000180006002400600007810000180013824501200015626000090027630000150028552011340030065000410143470000290147570000210150470000200152577301990154521773352025-07-15       2025    bl uuuu     u00u1 u    #d  a97804432355977 ahttps://doi.org/10.1016/B978-0-443-23560-3.00023-12DOI1 aMOLINA, Y. C.  aIn vitro “biotization” of plant tissue cultures and application of microbial inoculants.h[electronic resource]  c2025  ap. 433-448  ahroughout their lives, plants interact with environmental microorganisms at different levels of intimacy. The complexity of communications between the microbiome, plants, and the environmental environment can be considered a biological unit called a holobiont. In order to obtain microplants with desirable characteristics, high vigor, and freedom diseases, the tissue culture technique is a biotechnological alternative that allows the incorporation of new protocols, such as biotization, which can improve in vitro development and the acclimatization phase. The benefits attributed to biotization consist of promoting plant growth, modulating phytohormones, activation of the defense system, increasing rooting, and the survival rate of microplants. In addition to conditioning the plant to face the different stresses in ex vitro conditions. Thus, future research on the use of biotization will allow us to deepen and understand the interactions in the in vitro system. Therefore, this chapter addresses different perspectives on the in vitro tissue culture technique and future innovations in the area of plant biotechnology.  aPlant growth-promoting rhizobacteria1 aGÓMEZ SEPÚLVEDA, A. M.1 aRODRIGUES, F. A.1 aJESUS, E. da C.  tIn: KUMAR, A.; PANWAR, J. S.; QUEIJEIRO LÓPEZ, A. M.; KHARWAR, R. N. Microbial inoculants: soil dynamics and nutrient bioavailability: plant and soil microbiome. New York: Academic Press, 2025.