02751naa a2200337 a 450000100080000000500110000800800410001902400520006010000160011224501420012826000090027052017320027965000210201165000130203265000260204565000210207165000120209265300210210465300350212565300210216065300130218165300180219465300190221270000160223170000190224770000160226670000200228270000220230270000190232477300700234321769682025-07-07       2025    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.bcab.2025.1035942DOI1 aTIMM, T. G.  aImmunomodulatory properties of a β-glucan-rich bioflour of Bactris gasipaes shells colonized by Lentinula edodes.h[electronic resource]  c2025  aLentinula edodes β-glucans have shown potential in reducing lung injury caused by inflammation associated with severe acute respiratory syndrome (SARS). This study aimed to investigate the immunomodulatory properties related to lung inflammation, both in vitro and in vivo, of a high-β-glucan content bioflour derived from peach palm (Bactris gasipaes) shells colonized by Lentinula edodes (BPL). Neutrophils, L929, and A549 cells were treated (in vitro) with different doses (1, 10, 100 μg/mL) of BPL extracts (EBPL). The cells were subjected to cell viability assays, oxidative stress evaluation, and NO2 quantification. Male Swiss mice were divided into five groups (in vivo). Lung inflammation was induced via intranasal instillation of LPS (4 mg/kg), and the mice were treated orally with BPL. A control group received a vehicle or dexamethasone (0.5 mg/kg). Bronchoalveolar lavage (BAL) was collected to quantify total leukocytes, the mouse-to-lung weight ratio was calculated, and histological analysis was performed. Treatment with EBPL (in vitro) did not induce toxicity in cell lines and NO2 production decreased in neutrophils treated with the extracts; however, it did not reduce oxidative stress in A549 cells. A reduction in leukocytes was observed (in vivo) in BAL [11.43 × 105 (control), 9 × 105 (3 mg/kg), 3.33 × 105 (30 mg/kg), 1.43 × 105 (300 mg/kg) leukocytes/mL), histological infiltration, and a lower lung/mouse weight ratio [91.27 (control), 78.20 (3 mg/kg), 65.37 (30 mg/kg), 71.08 (300 mg/kg)]. This study highlights the nutraceutical potential of a β-glucan-rich food ingredient as an adjunct therapy for treating lung inflammation through dietary means, promoting human health and nutrition.  aBactris Gasipaes  aCogumelo  aDoença Respiratória  aLentinula Edodes  aPulmão  aCasca de pupunha  aImmunomodulation Lung diseases  aImunomodulação  aMushroom  aNutraceutical  aNutracêuticos1 aTIMM, T. G.1 aPITZ, M. A. C.1 aHELM, C. V.1 aALBERTON, M. D.1 aTAVARES, L. B. B.1 aMACHADO, I. D.  tBiocatalysis and Agricultural Biotechnologygv. 66, 103594, 2025.