02327naa a2200193 a 450000100080000000500110000800800410001902200140006002400520007410000250012624501690015126000090032052016660032965300360199570000170203170000240204870000160207277300450208821766172025-06-13       2025    bl uuuu     u00u1 u    #d  a1678-44997 ahttps://doi.org/10.1590/1678-4499.202401542DOI1 aGUIMARÃES. N. de F.  aGermination of Saccharum complex genotypes in response to inoculation using selected diazotrophic bacteria before and after a heat treatment.h[electronic resource]  c2025  aBreeding of new cultivars of sugarcane uses several species belonging to the Saccharum complex genus. In this study, a set of 20 genotypes from the Saccharum complex was assessed for their growth promotion during germination using a mixed inoculant containing five selected diazotrophs and their resistance to short heat treatment (52°C / 30 min). The percentage of germination was evaluated, leading to the identification of three response groups. Saccharum officinarum (group I) showed the highest germination percentage, succeeded by Erianthus arundinaceus genotypes (group II) and the other genotypes (group III). The mean germination time (MGT) varied among the genotypes and segregated the growth response into two groups. Saccharum officinarum only required less than 15 days to reach the MGT, whereas the other genera required more than two or three days during the initial growth process. Bacterial counting also differed between the groups, being effective for S. officinarum and other genotypes, improving the colonization of the plants 100–1,000 times higher than the control and heat treatments. Further comparison was made among eight genotypes, including one Saccharum spontaneum(US72-1319), one S. officinarum (IN845), and six E. arundinaceus. This comparison measured biomass accumulation and root parameters. The E. arundinaceus group demonstrated less susceptibility to the heat treatment, resulting in stimulation of the root dry mass of IJ76-381 and root parameters of this genotype, as well as IJ76-364, although not associated to the inoculation treatment. Inoculation applied to US72-1319 and IN845 enhanced only the Shoot/Root ratio.  aPlant growth promoting bacteria1 aALVES. G. C.1 aLORENZETI, I. J. de1 aREIS, V. M.  tBragantia, 84, e20240154, p. 1-16, 2025.