02139naa a2200253 a 450000100080000000500110000800800410001902400520006010000190011224501590013126000090029052013570029965000160165665300180167265300150169065300180170570000170172370000210174070000170176170000180177870000230179670000190181977300470183821764782025-06-06       2025    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s40858-025-00712-52DOI1 aBARBOSA, J. C.  aThe leafhopper Agallia albidula is a vector of a phytoplasma associated with “Huanglongbing”-like symptoms in citrus in Brazil.h[electronic resource]  c2025  aPhytoplasmas related to ‘Candidatus Phytoplasma pruni’, have been found in association with citrus “huanglongbing” (HLB) symptoms in Brazil. However, the lack of studies about phytoplasma vectors is a limiting factor for understanding epidemiological aspects and managing several diseases associated with phytoplasmas including citrus HLB. This study aimed to identify leafhopper species carrying phytoplasmas associated with HLB as potential insect vectors. Leafhoppers collected in citrus orchards with symptoms of HLB were individually confined on each healthy citrus plant for an inoculation access period (IAP) of 48 hr. After the IAP, each insect was tested for detection of phytoplasma. Evaluations of plants were performed one, four and six months after inoculation based on the presence of symptoms and phytoplasma in their tissues. Genomic fragments corresponding to the 16S rDNA were sequenced both for the citrus plants inoculated and their respective insects. A new strain related to ‘Candidatus Phytoplasma pruni’ (16SrIII-AD subgroup) was identified in the leafhopper Agallia albidula and citrus test plants inoculated. These findings indicate A. albidula as a vector of a phytoplasma associated with HLB-like symptoms in citrus, providing additional information that can contribute to the management of citrus HLB in Brazil.  aPhytoplasma  aInsect vector  aLeafhopper  aPlant disease1 aFERREIRA, J.1 aCOELHO, L. B. N.1 aECKSTEIN, B.1 aKREYCI, P. F.1 aBERGAMIN FILHO, A.1 aBEDENDO, I. P.  tTropical Plant Pathologygv. 50, 12, 2025.