02561naa a2200289 a 450000100080000000500110000800800410001902400460006010000210010624502320012726000090035952015250036865000180189365300240191165300270193565300270196265300220198965300200201170000220203170000230205370000250207670000230210170000170212470000140214170000190215577300970217421756132025-05-15       2025    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.56238/arev7n1-1532DOI1 aBENEVIDES, S. D.  aPotential of the essential oil of alpinia zerumbet (pers.) B.l. burtt & r. M. Sm. (Colonia) against alzheimer's disease, through antioxidant and anticholinesterase activity in vitro and in silico studies.h[electronic resource]  c2025  aThe plant Alpinia zerumbet, commonly known as "Colonia," is widely utilized in traditional medicine for addressing anxiety and hypertension, both of which are associated with oxidative damage in the brain. This study aimed to explore the antioxidant and anticholinesterase properties of the essential oil extracted from its leaves. Additionally, an in-silico investigation was carried out on several essential oil constituents concerning the acetylcholinesterase enzyme. The GC/MS analysis identified nineteen volatile compounds, mainly monoterpenoids (98.41%), particularly terpinen-4-ol, eucalyptol, y-terpinene, and sabinene, standing out as the major components. The anticholinesterase and radical-scavenging activities exhibited by A. zerumbet essential oil proved to be noteworthy when compared to standard drugs. In the molecular docking analysis, the compounds α-terpinene, β-pinene, eucalyptol, and terpinolene were found to bind to the same region as the standard galantamine binding site, suggesting their similar action. Conversely, o-cymene bound to a distinct region from the galantamine binding site, hinting at a potential synergistic effect with the co-crystallized inhibitor on AChE. Therefore, A. zerumbet essential oil, given its established pharmacological properties in the central nervous system (CNS) and the presence of antioxidant substances and acetylcholinesterase inhibitors, emerges as a promising candidate for more targeted pharmacological investigations related to Alzheimer's disease.  aFree radicals  aAncoragem Molecular  aCholinergic Hypothesis  aHipótese Colinérgica  aMolecular Docking  aRadicais Livres1 aPAULINO, F. W. V.1 aOLIVEIRA, D. P. DE1 aPEREIRA, R. de C. A.1 aSILVA, W. M. B. DA1 aALVES, D. R.1 aSILVA, J.1 aMARINHO, E. S.  tARACÊ - DIREITOS HUMANOS EM REVISTA, São José dos Pinhaisgv. 7, n. 1, p.2516-2534, 2025.