02309naa a2200241 a 450000100080000000500110000800800410001902400430006010000230010324501850012626000090031152015220032065000240184265000250186665000110189165000110190270000260191370000220193970000190196170000190198070000310199977300370203021728742025-02-17       2024    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.3390/v160608992DOI1 aOLIVEIRA, I. A. de  aGeminiviridae and Alphasatellitidae Diversity Revealed by Metagenomic Analysis of Susceptible and Tolerant Tomato Cultivars across Distinct Brazilian Biomes.h[electronic resource]  c2024  aThe diversity of Geminiviridae and Alphasatellitidae species in tomatoes was assessed via high-throughput sequencing of 154 symptomatic foliar samples collected from 2002 to 2017 across seven Brazilian biomes. The first pool (BP1) comprised 73 samples from the North (13), Northeast (36), and South (24) regions. Sixteen begomoviruses and one Topilevirus were detected in BP1. Four begomovirus-like contigs were identified as putative novel species (NS). NS#1 was reported in the semi-arid (Northeast) region and NS#2 and NS#4 in mild subtropical climates (South region), whereas NS#3 was detected in the warm and humid (North) region. The second pool (BP2) comprised 81 samples from Southeast (39) and Central–West (42) regions. Fourteen viruses and subviral agents were detected in BP2, including two topileviruses, a putative novel begomovirus (NS#5), and two alphasatellites occurring in continental highland areas. The five putative novel begomoviruses dis- played strict endemic distributions. Conversely, tomato mottle leaf curl virus (a monopartite species) displayed the most widespread distribution occurring across the seven sampled biomes. The overall diversity and frequency of mixed infections were higher in susceptible (16 viruses + alphasatellites) in comparison to tolerant (carrying the Ty–1 or Ty–3 introgressions) samples, which displayed 9 viruses. This complex panorama reinforces the notion that the tomato-associated Geminiviridae diversity is yet underestimated in Neotropical regions.  aSingle-stranded DNA  aSolanum lycopersicum  aTomate  aVírus1 aREIS, L. de N. A. dos1 aFONSECA, M. E. N.1 aMELO, F. F. S.1 aBOITEUX, L. S.1 aPEREIRA-CARVALHO, R. DE C.  tVirusesgv. 16, n. 6, 899, 2024.