01434naa a2200301 a 450000100080000000500110000800800410001902000220006002400370008210000180011924500890013726000090022630000130023549000420024852005260029065000300081665000190084665000140086565000110087965000110089065300160090165300330091770000230095070000210097370000150099470000240100977300990103321727972025-02-14       2024    bl uuuu     u00u1 u    #d  a978-1-0716-3485-17 a10.1007/978-1-0716-3485-1_52DOI1 aMORAIS, I. J.  aAgroinoculation of geminiviral infectious clones into plants.h[electronic resource]  c2024  ap. 65-70  a(Methods in molecular biology, 2724).  aMost geminiviruses are not transmitted by mechanical inoculation. Therefore, pathogenicity and plant–pathogen interaction studies rely on agroinoculation using infectious clones, which involves cloning the geminiviral genome in a binary vector (see previous chapter for details). A suspension containing the infectious clone inserted into Agrobacterium tumefaciens cells is then inoculated into plants, i.e., agroinoculated. Below is a simple protocol for agroinoculation of an infectious geminivirus clone into plants.  aAgrobacterium Tumefaciens  aPatogenicidade  aPatógeno  aPlanta  aVírus  aGeminivirus  aInteração planta patógeno1 aFERREIRA, Y. F. M.1 aNAKASU, E. Y. T.1 aNAGATA, T.1 aINOUE-NAGATA, A. K.  tIn: FONTES, E. P. B.; MÄKINEN, K.(ed.). Plant-virus interactions. London: Humana Press, 2024.