02030naa a2200241 a 450000100080000000500110000800800410001902400550006010000250011524501400014026000090028052012140028965300300150365300440153365300200157765300260159770000200162370000220164370000240166570000200168970000250170977300540173421712972025-01-08 2025 bl uuuu u00u1 u #d7 ahttps://doi.org/10.1016/j.carbpol.2024.1227692DOI1 aMENEZES, C. S. V. DE aExtraction of cell wall pectins and hemicellulose from agro-industrial wastesba sustainable alternative source.h[electronic resource] c2025 aThe efficient repurposing of agro-industrial waste has significantly enhanced the utilization of food resources. This study aims to propose a methodology for extracting cell wall polysaccharides from residues of mango (Mangifera indica), passion fruit (Passiflora edulis), and cashew (Anacardium occidentale). Polysaccharide fractions were obtained through sequential extraction protocols involving water, cyclohexane-trans-1,2-diamine tetracetate (CDTA), sodium carbonate:CDTA, and potassium hydroxide. These fractions were categorized as water-soluble pectin (WSP), CDTA-soluble pectin (CSP), sodium carbonate-soluble pectin (SSP), and hemicellulose (HC), respectively. Each polysaccharide fraction was characterized by Nuclear Magnetic Resonance (NMR) spectroscopy and Gel Permeation Chromatography (GPC). Monosaccharide composition was determined using Gas Chromatography-Mass Spectrometry (GC–MS). NMR spectra of WSP, CSP, and SSP fractions exhibited characteristic pectin features, while the HC fraction primarily comprised hemicellulose. Consequently, the proposed methodology demonstrates potential as a standardized protocol for the extraction of pectin and hemicellulose from various food sources. aPolysaccharide extraction aRepurposing of agro-industrial residues aTropical fruits aWaste reuse potential1 aSOUSA, A. C. C.1 aSILVA, L. M. A. e1 aRODRIGUES, T. H. S.1 aBRITO, E. S. de1 aRICARDO, N. M. P. S. tCarbohydrate Polymersgv. 347, Jan. 122769, 2025.