02122naa a2200277 a 450000100080000000500110000800800410001902400520006010000240011224501550013626000090029152012730030065000140157365000250158765000150161265000220162765000220164970000250167170000230169670000170171970000260173670000240176270000160178670000210180277300210182321667472024-08-23       2024    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s10570-024-06115-02DOI1 aSIMPLICIO, E. da S.  aEnzymatic hydrolysis of sugarcane and oil palm residues after hydrothermal pretreatmentbthe attainment of cellulose nanofbers.h[electronic resource]  c2024  aIn the present work, the use of enzymatic hydrolysis to obtain cellulose nanofbers composed of purifed cellulose from sugarcane bagasse (SCB) and oil palm empty fruit bunches (OPEFBs)was addressed. First, the cellulose was purifed by applying hydrothermal treatments conducted at various temperatures (180 and 200 °C) and times (10, 20and 30 min) and then 2% (m/v) sodium chlorite treatment. Then, enzymatic hydrolysis was conducted for 24, 48 or 72 h using cellulase from Trichoderma reesei at 50 °C and pH=5.0 to isolate the cellulose nanofbers. The composition, morphology, crystallinity, thermal stability and zeta potential characteristics were evaluated for each cellulosic material. The crystallinity index increased signifcantly after hydrothermal pretreatment and bleaching from 42.6% to 87.3% and from 42.5% to 86.1% for SCB and OPEFB, respectively. In addition, the obtained pulps showed better thermal stability than residues when used as feedstock. Enzymatic hydrolysis was efective at extracting cellulose nanofbers, as long as the reaction time was controlled to less than 48 h, to avoid compromising the crystallinity and thermal stability of the isolated nanostructures. The enzymatic pathway is a promising alternative to the use of acid catalysts.  aCellulose  aEnzymatic hydrolysis  aNanofibers  aOil palm products  aSugarcane bagasse1 aBRITO NETA, M. de S.1 aBRITO, G. F. da S.1 aANDREANI, L.1 aCARVALHO, F. B. de P.1 aRODRIGUES, D. de S.1 aMACHADO, F.1 aVALADARES, L. F.  tCellulose, 2024.