02572naa a2200265 a 450000100080000000500110000800800410001902200140006002400550007410000220012924501390015126000090029030000110029952017730031065300150208365300220209865300230212070000200214370000190216370000190218270000230220170000190222470000230224377300400226621664892024-10-11       2024    bl uuuu     u00u1 u    #d  a0309-17407 ahttps://doi.org/10.1016/j.meatsci.2024.1096212DOI1 aSARTORI, A. G. O.  aLipidome and metabolome profiling of longissimus lumborum beef with different ultimate pH and postmortem aging.h[electronic resource]  c2024  a1 - 10  aA B S T R A C T The objective o0 this exploratory study was to assess the changes on lipidome and metabolome profiling of Longissimus lumborum bull muscle with di00erent ultimate pH (pHu) and aging periods. The bull muscles classified as normal, intermediate, or high pHu were collected from a Brazilian commercial slaughterhouse, cut into steaks, individually vacuum-packaged, and aged 0or 3 days (3-d) or 21 days (21-d) at 2 ±C. Muscle extracts were analyzed for the profiles of both lipids, by mass spectrometry (via direct fow-injection), and metabolites, by nuclear magnetic resonance, with downstream multivariate data analysis. As major results, pairwise comparisons identified C12:0 and C14:0 acylcarnitines as potential biomarkers o0 the intermediate pHu-muscle, which are related to lipid catabolism 0or alternative energy metabolism and indicate less protein breakage postmortem. Interestingly, the concentration of arginine at early postmortem aging (3-d) may infuence the previously reported improved tenderness in normal and high pHu-muscles. Moreover, upregulation of fumarate, formate, and acetate with increased pHu muscle at 21-d aging indicate more intense tricarboxylic acid cycle, amino acid degradation, and pyruvate oxidation by reactive oxygen species, respectively. These three compounds (fumarate, formate, and acetate) discriminated statistically the muscle with high pHu at 21-d aging. The normal pHu-muscle showed higher concentrations of glycogenolysis and glycolysis metabolites, including glucose, mannose, and pyruvate. Hence, our results enhance knowledge of postmortem biochemical changes of bee0 within different pHu groups aged up to 21 days, which is essential to understand the mechanisms underpinning bull meat quality changes.  aLipidomics  aMeat biochemistry  aShotgun lipidomics1 aANTONELO, D. S.1 aRIBEIRO, G. H.1 aCOLNAGO, L. A.1 aBALIEIRO, J. C. C.1 aDELGADO, E. F.1 aCASTILLO, C. J. C.  tMeat Sciencegv. 217, 109621, 2024.