02443naa a2200337 a 450000100080000000500110000800800410001902400360006010000170009624501880011326000090030130000100031052014100032065000120173065000390174265300250178165300200180665300160182665300240184270000200186670000190188670000210190570000180192670000190194470000260196370000190198970000250200870000200203370000220205377300300207521650712024-06-24       2024    bl uuuu     u00u1 u    #d7 a10.1007/s00425-024-04460-92DOI1 aTIEPO, A. N.  aInfuence of plant growth‑promoting bacteria on leaf carbon and nitrogen metabolism of two drought‑stressed neotropical tree speciesba metabolomic approach.h[electronic resource]  c2024  a13 p.  aAbstract Deforestation of Atlantic Forest has caused prolonged drought events in the last decades. The need for reforestation is growing, and the development of native seedlings that are more tolerant to drought stress is necessary. A biotechnological tool that improves plant tolerance is the use of plant growth-promoting bacteria (PGPB) as inoculants. Two species of PGPB were inoculated in drought-stressed seedlings of two neotropical tree species that have been used in environmental restoration programs: Cecropia pachystachya and Cariniana estrellensis. Biometrical, physiological, and metabolomic parameters from carbon and nitrogen pathways were evaluated. We found that the PGPB positively infuenced photosynthesis and growth parameters in both trees under drought. The enzymes activities, the tricarboxylic acid cycle intermediates, the amino acids, and protein contents were also infuenced by the PGPB treatments. The results allowed us to fnd the specifc composition of secondary metabolites of each plant species. This study provides evidence that there is not a single mechanism involved in drought tolerance and that the inoculation with PGPB promotes a broad-spectrum tolerance response in Neotropical trees. The inoculation with PGPB appears as an important strategy to improve drought tolerance in Atlantic Forest native trees and enhance environmental restoration programs’ success.  aCarbono  aEstimulante de Crescimento Vegetal  aAssociative bacteria  aAtlantic forest  aMetabolomic  aPlant-microorganism1 aCOUTINHO, I. D.1 aMACHADO, G. O.1 aCALZAVARA, A. K.1 aHERTEL, M. F.1 aPIMENTA, J. A.1 aOLIVEIRA, A. L. M. de1 aCOLNAGO, L. A.1 aMERTZ-HENNING, L. M.1 aOLIVEIRA, H. C.1 aSTOLF-MOREIRA, R.  tPlantagv. 260, 31, 2024.