02077naa a2200313 a 450000100080000000500110000800800410001902400540006010000170011424501680013126000090029952011040030865000170141265000110142965000120144065300240145265300170147665300270149365300260152070000190154670000220156570000180158770000140160570000160161970000200163570000170165570000240167277300670169621643072025-02-07       2023    bl uuuu     u00u1 u    #d7 ahttp://dx.doi.org/10.1007/s13399-023-05040-92DOI1 aCLARO, F. C.  aCharacterisation of laccase-mediated lignin polymerisationbimplications for molecular weight, thermal stability, and electrical properties.h[electronic resource]  c2023  aKraft lignin was oligomerised using a bulk laccase solution in varying amounts of buffer and ethanol solvents. The activity of laccase and kinetic parameters was analysed by adjusting the buffer pH and ethanol:buffer ratio. The qualitative weighted average molecular weight (Mw) distribution showed significant variation when ethanol (40:60) solvent was used. GPC analysis confirmed the presence of fractions with higher molecular weight, where the Mw after 30 min was 7859, compared to 90 min in the buffer medium, where M w was only 1779. These condensed units exhibited lower antioxidant values, characteristic of a polymerisation reaction. Py-GC/MS analysis demonstrated that the sample with the highest molecular weight displayed increased G-type lignin units and higher levels of polysaccharides residue compared to the original kraft lignin. The addi- tion of ethanol as a green solvent, mixed with buffer, resulted in a 4.4-fold increase in M w compared to buffer alone. These findings highlight the advantageous role of ethanol as a medium to obtain lignin units with high molecular weight.  aAntioxidants  aEtanol  aLignina  aElectric conduction  aKraft lignin  aLaccase polymerisation  aLignin polymerisation1 aLIMA, G. G. de1 aLIMA, T. A. M. de1 aHANSEL, F. A.1 aMATOS, M.1 aAVELINO, F.1 aOLIVEIRA, D. R.1 aLOMONACO, D.1 aMAGALHAES, W. L. E.  tBiomass Conversion and Biorefinerygv. 15, p. 2479-2494, 2025.