03529naa a2200373 a 450000100080000000500110000800800410001902400520006010000220011224501680013426000090030252024450031165000130275665000160276965000120278565000080279765300170280565300100282265300180283265300150285065300190286565300180288470000210290270000180292370000180294170000230295970000220298270000210300470000240302570000200304970000210306970000170309077300480310721641732024-05-27       2024    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1186/s12864-024-09983-92DOI1 aDAL PIZZOL, M. S.  aDifferential expression of miRNAs associated with pectoral myopathies in young broilersbinsights from a comparative transcriptome analysis.h[electronic resource]  c2024  aAbstract: Introduction: White Striping (WS) and Wooden Breast (WB) pectoral myopathies are relevant disorders for con- temporary broiler production worldwide. Several studies aimed to elucidate the genetic components associated with the occurrence of these myopathies. However, epigenetic factors that trigger or differentiate these two condi- tions are still unclear. The aim of this study was to identify miRNAs differentially expressed (DE) between normal and WS and WB-affected broilers, and to verify the possible role of these miRNAs in metabolic pathways related to the manifestation of these pectoral myopathies in 28-day-old broilers. Results: Five miRNAs were DE in the WS vs control (gga-miR-375, gga-miR-200b-3p, gga-miR-429-3p, gga-miR- 1769-5p, gga-miR-200a-3p), 82 between WB vs control and 62 between WB vs WS. Several known miRNAs were associated with WB, such as gga-miR-155, gga-miR-146b, gga-miR-222, gga-miR-146-5p, gga-miR- 29, gga-miR-21-5p, gga-miR-133a-3p and gga-miR-133b. Most of them had not previously been associated with the development of this myopathy in broilers. We also have predicted 17 new miRNAs expressed in the broilers pectoral muscle. DE miRNA target gene ontology analysis enriched 6 common pathways for WS and WB compared to control: autophagy, insulin signaling, FoxO signaling, endocytosis, and metabolic pathways. The WS vs control contrast had two unique path- ways, ERBB signaling and the mTOR signaling, while WB vs control had 14 unique pathways, with ubiquitin-mediated proteolysis and endoplasmic reticulum protein processing being the most significant. Conclusions: We found miRNAs DE between normal broilers and those affected with breast myopathies at 28 days of age. Our results also provide novel evidence of the miRNAs role on the regulation of WS and in the differentiation of both WS and WB myopathies. Overall, our study provides insights into miRNA-mediated and pathways involved in the occurrence of WS and WB helping to better understand these chicken growth disorders in an early age. These findings can help developing new approaches to reduce these complex issues in poultry production pos- sibly by adjustments in nutrition and management conditions. Moreover, the miRNAs and target genes associated with the initial stages of WS and WB development could be potential biomarkers to be used in selection to reduce the occurrence of these myopathies in broiler production.  aChickens  aEpigenetics  aGalinha  aRNA  aEpigenética  aPeito  aRNAs pequenos  aSmall RNAs  aWhite striping  aWooden breast1 aIBELLI, A. M. G.1 aCANTAO, M. E.1 aCAMPOS, F. G.1 aOLIVEIRA, H. C. de1 aPEIXOTO, J. de O.1 aFERNANDES, L. T.1 aTAVERNARI, F. de C.1 aMORES, M. A. Z.1 aBASTOS, A. P. A.1 aLEDUR, M. C.  tBMC Genomicsgv. 25, n. 104, p. 1-14, 2024.