01812nam a2200313 a 450000100080000000500110000800800410001910000220006024501330008226001560021552007790037165000320115065000160118265000130119865000250121165000390123665000130127565000140128865000110130265000110131365300280132470000210135270000150137370000180138870000230140670000220142970000230145170000240147421635962024-04-15       2023    bl uuuu     u00u1 u    #d1 aLIMA, R. R. P. N.  aAnálise de sequenciamento revela que pimentão é um novo hospedeiro potencial de potato leafroll virus.h[electronic resource]  aIn: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 53., Brasília, DF, 2023. Anais... Brasília, DF: Sociedade Brasileira de Fitopatologia, 2023. p. 699.c2023  aSequencing analysis reveals that peppers is a potencial new host for potato leafroll virus. Tomato (Solanum lycopersicum) and pepper (Capsicum annuum) are among the most important vegetable crops worldwide. However, these crops are severely affected by multiple viral diseases. To survey the diversity of viral species infecting tomato and pepper plants in different regions of Brazil, symptomatic plants were sampled from the Federal District, Mato Grosso, Pernambuco, Goiás and São Paulo states. Pools of leaves were used for semi-purification of virus particles through a method of differential centrifugation. The pellets were resuspended and used for total RNA extraction, followed by standard Illumina library preparation with ribosomal RNA deletion, and sequencing.  aCapsicum annuum var. annuum  aPolerovirus  aPotatoes  aSolanum lycopersicum  aSolanum tuberosum subsp. tuberosum  aTomatoes  aPimentão  aTomate  aVírus  aExtração de RNA total1 aNAKASU, E. Y. T.1 aBLAWID, R.1 aVARGAS, O. R.1 aSOUSA, B. M. C. de1 aPEREIRA, W. de S.1 aFERREIRA, Y. F. M.1 aINOUE-NAGATA, A. K.