01436naa a2200325 a 450000100080000000500110000800800410001902200140006002400570007410000150013124501270014626000090027352003690028265000300065165300290068165300340071065300260074465300320077065300190080265300180082165300320083965300120087165300370088365300190092070000200093970000180095970000280097770000300100577300750103521633032024-04-05       2024    bl uuuu     u00u1 u    #d  a1984-70337 ahttp://dx.doi.org/10.1590/1984-70332024v24n1a012DOI1 aKOLTUN, A.  aMicrosatellite markers in maizebchallenges and guidelines for implementing multiplex SSR analyses.h[electronic resource]  c2024  aThis study tested two previously constructed simple sequence repeat (SSR) sets containing ten primer pairs each (ten-plex) in 1142 maize genotypes. Here, we describe the challenges faced when implementing this microsatellite-based genotyping protocol in our laboratory and possible ways to overcome them, hopefully aiding other novice research teams in this field.  aPolymerase chain reaction  aFluorophore interference  aInterferência de fluoróforo  aManual allele scoring  aMarcadores microssatélites  aMilho tropical  aMultiplex PCR  aPontuação alélica manual  aPull-up  aReação em cadeia da polimerase  aTropical maize1 aSILVA, P. A. da1 aTORRES, I. Y.1 aBONIFÁCIO-ANACLETO, F.1 aYASSITEPE, J. E. de C. T.  tCrop Breeding and Applied Biotechnologygv. 24, n. 1, e46392411, 2024.