02312naa a2200253 a 450000100080000000500110000800800410001902400520006010000220011224501180013426000090025252015650026165000160182665000180184265000140186065300310187465300110190570000170191670000180193370000200195170000200197170000150199177300520200621628462024-03-14       2024    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s40858-024-00647-32DOI1 aFAJARDO, T. V. M.  aAnalyzes of mealybug (Pseudococcus longispinus) virome reveal grapevine viruses diversity.h[electronic resource]  c2024  aThe long-tailed mealybug, Pseudococcus longispinus, is an important insect pest in grapevine growing areas in several countries, including Brazil. Metagenomic analysis of nucleic acids extracted from insect vectors makes it possible to study the diversity of insect viruses in addition to plant pathogenic viruses. In this study, insects (Ps. longispinus) were collected, and pooled throughout a plot of virus disease symptomatic vines, cultivated in growing beds, and analyzed by high throughput sequencing (HTS). The complete genome of grapevine leafroll-associated virus 2 and 3 (GLRaV-2 and -3) and a partial sequence of grapevine virus A (GVA) with two complete ORFs (coat protein and RNA-binding protein) were assembled from mealybug extracts and exhibited high nucleotide identities, up to 99%, with previously characterized homologous Brazilian isolates from grapevines. This information was validated by the detection of these viruses in the original symptomatic vines (N=76), from where mealybugs were collected, equivalent to an incidence of 34.2%, 89.5% and 36.8% for GLRaV-2, GLRaV-3 and GVA, respectively. Although one of these viruses is not transmitted by mealybugs (GLRaV-2), prospective of plant viruses infecting grapevine plants by analyzing the metagenome of insects could represent a relevant alternative to improve monitoring of viral diseases aiming at the management and control of viral diseases in vineyards or cultivation fields. This work is the first analysis of the Ps. longispinus virome in Brazil focusing on grapevine viruses.  aAmpelovirus  aClosterovirus  aVitivirus  aHigh throughput sequencing  aVector1 aGRYNBERG, P.1 aTOGAWA, R. C.1 aSILVA, J. M. F.1 aSILVA, F. N. da1 aNICKEL, O.  tTropical Plant Pathology, [online], March 2024.