02353naa a2200301 a 450000100080000000500110000800800410001902200140006002400530007410000240012724501410015126000090029252014540030165000200175565000090177565000250178465000220180965000100183165000130184165300190185465300200187365300250189370000250191870000240194370000160196770000220198377300460200521626832024-03-07       2024    bl uuuu     u00u1 u    #d  a2352-51347 ahttps://doi.org/10.1016/j.aqrep.2024.1020002DOI1 aRODRIGUES, A. P. O.  aFeeding rate and feeding frequency during the grow-out phase of tambaqui (Colossoma macropomum) in earthen ponds.h[electronic resource]  c2024  aAdequate feeding protocols are extremely important for the technical and economic feasibility of fish production systems. Two feeding rates, 3% and 4% body weight per day (3%BW and 4%BW), and two feeding frequencies, twice and three times a day, were tested during 84 days at the initial grow-out phase of tambaqui (94.5 ± 6.4 g) in earthen ponds. No mortalities or difference in growth between treatments were observed during the trial. Total feed intake and feed conversion ratio were low in the fish fed at 3%BW. Fat deposition in the fish carcass and concentration of glycogen and lipid vacuoles in the hepatocytes were also low in fish fed at 3%BW. Plasma cholesterol and triglycerides were higher in the fish fed three times a day at 4%BW. Increased concentration of malonaldehyde was observed in the carcass of fish fed three times a day at 4%BW at the end of the storage period. Fish fed twice a day presented higher total feed intake per meal than fish fed three times a day, demonstrating that tambaqui was able to regulate the feed intake to benefit from a low feeding frequency. No difference was observed in pond phytoplankton and zooplankton concentration or sediment chemistry. Fish fed at 3%BW presented higher stomach content weight, demonstrating the contribution of the natural food as a complementary nutrition to tambaqui. Based on the results, tambaqui of 95–350 g reared in earthen ponds may be fed twice a day at 3%BW.  aFeed conversion  aFish  aColossoma Macropomum  aNutrição Animal  aPeixe  aTambaqui  aAmazonian fish  aFeed management  aNatural productivity1 aFREITAS, L. E. L. de1 aMACIEL-HONDA, P. O.1 aLIMA, A. F.1 aLIMA, L. K. F. de  tAquaculture Reportsgv. 35, 102000, 2024.