01800nam a2200301 a 450000100080000000500110000800800410001910000220006024501050008226001410018730000120032852008420034065000110118265000240119365000260121765000220124365000130126565000120127865000230129065000240131365000250133765300260136270000210138870000230140970000260143270000200145870000200147821623172024-03-12       2023    bl uuuu     u00u1 u    #d1 aSANTOS, M. M. dos  aMeloidogyne incognita control in cotton mediated host-induced gene silencing.h[electronic resource]  aIn: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 53., 2023, Brasília, DF. Anais... Brasília, DF: Sociedade Brasileira de Fitopatologiac2023  ap. 582.  aIn the present work we used a Host-Induced Gene Silencing (HIGS) strategy employing RNA interference (RNAi) mechanisms in order to silence RKN essential genes involved in development (cathepsin L cysteine proteinase - MiCPL), metabolism (isocitrate lyase - MiICL), mRNA processing (splicing factor - MiSF) and parasitism (nematode effector Mi16D-10). We staked all four RKN genes into a single construct (dsMINC2), driven by the pUceS8.3 constitutive soybean promoter, in order to control the nematode reproduction in transgenic cotton plants developed from an RKN-susceptible cultivar (BRS372). Transgenic dsMinc2-T2 plants infected with Meloidogyne incognita showed a significant reduction in the number of eggs/g.root -1 (73 - 93%) as well as in the reproduction factor (80-93%), compared with the susceptible non-transgenic cultivar.  aCotton  aRoot-knot nematodes  aSmall interfering RNA  aTransgenic plants  aAlgodão  aDoença  aGossypium Hirsutum  aPlanta Transgênica  aProdução Agrícola  aNematóide das galhas1 aSÁ, M. E. L. de1 aLEMES, S. V. da R.1 aCAVALCANTE, R. dos S.1 aMORGANTE, C. V.1 aSA, M. F. G. de