02429naa a2200277 a 450000100080000000500110000800800410001902400520006010000190011224501320013126000090026352015510027265000150182365000200183865300270185865300230188565300240190865300300193265300150196265300220197765300280199970000160202770000160204370000220205977300700208121621372024-03-12       2023    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.bcab.2023.1026822DOI1 aSERBENT, M. P.  aGrowth, laccase activity and role in 2,4-D degradation of Lentinus crinitus (L.) Fr. in a liquid medium.h[electronic resource]  c2023  aThis study aimed to evaluate the role of L. crinitus laccase in the biotransformation of 2,4-D pesti- cide and the adsorption capacity and/or accumulation in the fungal biomass. Preliminary tests were carried out in liquid medium containing L. crinitus to detect the maximum concentration of 2,4-D in which the mycelium developed (5.025 g L−1). The growth and laccase activity for L. crinitus were analyzed using a complete factorial experimental design with variations in concen- trations of glucose (16.65–33.35 g L−1), malt extract (0.825–9.175 g L−1), and 2,4-D (0.01–5 gL-1), and the mycelial samples were evaluated by SEM-EDS. The ability of L. crinitus to remove 2,4-D after 28 days was demonstrated by a decrease in concentrations of 2,4-D by up to 15.7% more than control treatments and occurred with both low (&lt2 g L−1) and high (&gt10 g L−1) glu- cose consumption conditions, reaching biomass concentrations below 2 g L−1, and presenting lac- case activity between 3 and 6 g L−1. The laccase activity of the fungus depends on the initial amount of glucose and 2,4-D (up to 18 g L−1 and 4 g L−1). Lower concentrations of 2,4-DCP (0.01–2.70 mg L−1) may be an indicative of L. crinitus as its transformation agent. The presence of chlorine in the fungal mycelium (up to 4.0%) may represent its adsorption and/or accumulation by L. crinitus. L. crinitus can be described as a biotransformator of 2,4-D and 2,4-DCP through lac- case activity, highlighting its potential for treatment of agricultural effluents.  aHerbicides  aWhite-rot fungi  aBioprocessos fúngicos  aEffluent treatment  aFungal bioprocesses  aFungo de podridão branca  aHerbicidas  aLentinus crinitus  aTratamento de efluentes1 aTIMM, T. G.1 aHELM, C. V.1 aTAVARES, L. B. B.  tBiocatalysis and Agricultural Biotechnologygv. 50, 102682, 2023.