02087naa a2200349 a 450000100080000000500110000800800410001902400570006010000180011724501110013526000090024652010860025565000100134165000140135165000120136565300260137765300170140365300220142065300180144265300160146065300160147670000200149270000190151270000220153170000210155370000180157470000190159270000200161170000240163170000210165577300610167621615622024-02-02       2024    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.jinorgbio.2023.1124412DOI1 aMAGARELLI, G.  aA voltammetric peptide biosensor for Cu2+ metal ion quantification in coffee seeds.h[electronic resource]  c2024  aA prion-derived copper(II)-binding peptide was assembled onto a gold electrode for the building of a voltammetric biosensor for measuring the Cu2+ metal ion in biological samples. The chosen sequence was HCVNITKQHTVTTTT-NH2, with an appended cysteine residue for binding to the gold surface as a self-assembled monolayer and a histidine residue as the anchorage point for copper(II) complexation. The biosensor showed a linear range of 10-7 to 10-6 M with an 8.0 x 10-8 M detection limit and a 1.0 x 10-7 M quantification limit, with good precision, trueness, and absence of matrix effect. The quantification of Cu2+ was performed in the presence of other transition metal ions, such as Zn2+, Cd2+, Fe2+, or Ni2+, which indicates the excellent selectivity of the biosensor. When the modified electrode was applied for measuring copper(II) in calcined coffee seeds, a difference in copper amount was observed between two Coffea arabica cultivars that were submitted to a treatment with a copper-based antifungal, showing the applicability of the biosensor in the agricultural field.  aCafé  aPeptídeo  aSemente  aBiossensor peptídico  aCoffee seeds  aPeptide biosensor  aPrion peptide  aVoltametria  aVoltammetry1 aSILVA, J. G. da1 aRIBEIRO, C. L.1 aFREITAS, T. V. de1 aRODRIGUES, M. A.1 aGIL, E. de S.1 aMARRACCINI, P.1 aSOUZA, J. R. de1 aCASTRO, C. S. P. de1 aBEMQUERER, M. P.  tJournal of Inorganic Biochemistrygv. 251, 112441, 2024.